Edvotek Series 200 Experiments. For 10 Restriction Digestions for 10 Gels. Time Required after enzyme digestion, gel preparation and electrophoresis: Staining -- 15 min.; Destaining -- 30 min.
The DNA from bacteriophage lambda is a well-characterized linear molecule containing six recognition sites for Eco RI (5 distinct sites; 2 are very close in size). This experiment introduces site-specific restriction endonuclease digestions of Eco RI for analysis by agarose gel electrophoresis.
REQUIREMENTS: Horizontal gas electrophoresis apparatus; D.C. power supply; automatic micropipet with tips; 65°C waterbath; visualization system (white light); pipet pump or bulb; hot gloves and safety goggles; distilled or deionized water; ice; microwave oven or hot plate; balance; large weigh boats for destaining; photodocumentation system (optional); floating racks for microtubules. Some components require freezer storage at -20°C upon arrival.
CONTENTS: Lambda DNA; Dryzymes™-Eco RI; Restriction Enzyme Reaction Buffer; 10x Gel Loading Solution; UltraSpec-Agarose™ Powder; Practice Gel Loading Solution; Concentrated 50x Electrophoresis Buffer; Methylene Blue Plus™; DNA Blue InstaStain™; 1ml pipets; 100ml Graduated Packaging Cylinder; Microtipped Transfer Pipets; Microtest Tubes with Attached Caps; Semi-log Graph Paper Template; Complete instructions, background information, and study questions.
