Edvotek. Series 200 Experiments. For 10 Transformations and controls. Time Required: Transformation -- 45 min. Plating -- 5 min. Incubation -- overnight. Transformation Efficiency -- 15 min.

Transformation is of central importance in molecular cloning since it ultimately allows for the selection, propagation, expression and purification of a gene. Positive selection for cells containing plasmid DNA is accomplished by growth on antibiotic plates. This experiment includes Transformation Lyphocells™, an exclusive bacterial transformation system which eliminates the need for dry ice shipping and -70°C storage of competent cells.

REQUIREMENTS: Automatic micropipet (5-50µl) and tips; two waterbaths; incubation oven; pipet pumps or bulbs; ice; hot plate or microwave oven; hot gloves and goggles; latex or vinyl gloves; marking pens. Some components require freezer storage at -20°C upon arrival.

CONTENTS: Lyphocells™; Plasmid DNA; Induction Buffer; Reconstitution Buffer; Ampicillin Antibiotic; ReadyPour™ Luria Broth Agar; Luria Broth Media for Recovery; Petri Plates, 60mm and 100mm; Wrapped 10ml pipets; Sterile Inoculating Loops; Microtest Tubes with Attached Caps; Calcium Chloride; Sterile Calibrated Transfer Pipets; Toothpicks; Complete instructions, background information, and study questions.