Edvotek Series 200 Experiments. For 10 Transformations and controls. Time Required: Transformation -- 45 min.; Plating -- 5 min.; Incubation -- overnight; Transformation Efficiency -- 15 min.

Kit includes E. coli Transformation Lyphocells™, which eliminates the need for dry ice shipping and -70°C storage.

In this experiment, the transformants acquire antibiotic resistance and exhibit a blue color due to the incorporation and expression of §-galactosidase and ampicillin resistance genes. IPTG is not required, since pGAL™ contains the intact §-galactosidase gene.

REQUIREMENTS: Automatic micropipet (5-50µl) and tips; two waterbaths; incubation oven; pipet pumps or bulbs; ice; hot plate or microwave oven; hot gloves and safety goggles; latex or vinyl gloves; marking pens. Some components require freezer storage at -20°C upon receipt.

CONTENTS: Lyphocells™; Plasmid DNA; Induction Buffer; Reconstitution Buffer; Ampicillin Antibiotic; X-Gal in Solvent; ReadyPour™ Luria Broth Agar; Luria Broth Media for Recovery; 40 Petri Plates (60mm); Sterile Calibrated Transfer Pipets; Wrapped 10ml pipets; Sterile Inoculating Loops; Microtest Tubes with Attached Caps; Complete instructions, background information, and study questions.