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Investigation 8: Transformation of E. coli with Green Fluorescent Protein (GFP)


Edvotek.  In this experiment, students will explore the biological process of bacterial transformation using E. coli and plasmid DNA. At the end of the activity, students will have experience observing and analyzing acquired traits (ampicillin resistance and fluorescence) as exhibited by transformed bacterial cells.Genetic Engineering, 

For 10 Lab Stations. Set up and plating 50 minutes. Incubation overnight. Transformation efficiency 15 minutes.

To download instructons go to:

 Kit includes: instructions, BactoBeads™ E. coli GFP Host, supercoiled pFluoroGreen™ plasmid DNA, ampicillin, IPTG, CaCl2, Growth Additive, ReadyPour™ Luria Broth Agar (sterile), Luria Broth Medium for Recovery (sterile), petri plates (small), petri plates (large), plastic microtipped transfer pipets, wrapped 10 ml pipet (sterile), toothpicks (sterile), inoculating loops (sterile), microcentrifuge tubes 

All you need: automatic micropipet (5-50 µl) and tips, two waterbaths (37ºC and 42ºC), thermometer, incubation oven (37ºC), pipet pumps or bulbs, ice, marking pens, bunsen burner, hot plate or microwave, hot gloves, long wave UV light

Storage: Some Components Require Refrigerator and Freezer Storage - See more at:

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