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Lab 2: Principles Of Enzyme Catalysis


Edvotek Classic AP Biology Lab.  This easy and safe experiment allow your students to learn about enzyme catalysis, the nature of enzyme action and protein structure-function relationships. Students will perform an enzyme assay and determine the rate of the enzymatic reaction. This experiment uses a safer system that eliminates the need for sulfuric acid and potassium permanganate.

For 10 Lab groups.  Requires 30 - 45 minutes.

To download instructions go to:

Kit includes: instructions, catalase solution, 1.2% hydrogen peroxide, phosphate buffer, assay reagent, acidification solution, color enhancer, color developer.

All you need: visible wavelength spectrophotometer, timer, pipet pumps, test tubes & racks, 1, 5 & 10 ml pipets, beakers, graph paper, distilled water, ice.

Storage: Some Components Require Refrigerator & Freezer Storage 

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