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Lab 2: Principles Of Enzyme Catalysis

APB282

Edvotek Classic AP Biology Lab.  This easy and safe experiment allow your students to learn about enzyme catalysis, the nature of enzyme action and protein structure-function relationships. Students will perform an enzyme assay and determine the rate of the enzymatic reaction. This experiment uses a safer system that eliminates the need for sulfuric acid and potassium permanganate.

For 10 Lab groups.  Requires 30 - 45 minutes.

To download instructions go to:  http://www.edvotek.com/site/pdf/282.pdf

Kit includes: instructions, catalase solution, 1.2% hydrogen peroxide, phosphate buffer, assay reagent, acidification solution, color enhancer, color developer.

All you need: visible wavelength spectrophotometer, timer, pipet pumps, test tubes & racks, 1, 5 & 10 ml pipets, beakers, graph paper, distilled water, ice.

Storage: Some Components Require Refrigerator & Freezer Storage 

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