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Lab 4: Plant Pigment Chromatography And Photosynthesis


Edvotek Classic AP Biology Lab.  Chromatographic separation is performed by thin layer chromatography. Key reagents are included to demonstrate photosynthesis.

For 10 Lab Groups.  Requires 1.75 hours.  (2 Lab Periods)

To download instructions go to:

Kit includes: instructions, chloroplast extraction; dilution buffers, reducing reagent, DCIP, standard pigments, chromatography solvent, Sarkosyl, thin layer cellulose chromatography plate, microliter capillary pipets, tubes and transfer pipets.

All you need: visible wavelength spectrophotometer, clinical benchtop centrifuge, blender, flood light, calculator, timer, test tubes; racks, beakers, 1 & 5 ml pipets, pipet pumps, metric rulers, cheesecloth, 95-100% isopropanol, distilled water, fresh spinach, ice.

Storage: Some Components Require Freezer Storage

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