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Lab 6: Transformation Of E. Coli With Pgal™ (Blue Colony)

BT221

Edvotek  Classic AP Biplogy Lab. In this experiment, your students will develop an understanding of bacterial transformation by plasmid DNA by introducing an opportunity to observe an acquired phenotypic trait of the transformed bacterial cells. The presence of blue bacterial colonies visually demonstrates the expression of a specific gene for the Lac+ phenotype.

No IPTG used in this experiment.

For 10 Lab Groups. Complete in 50 minutes & grow overnight.

To download instructions go to:  http://www.edvotek.com/site/pdf/221.pdf

Kit includes: instructions, BactoBeads™, plasmid DNA, buffer, media, ampicillin, X-Gal, ReadyPour™ agar, petri dishes, sterile pipettes, loops and microtubes.

All you need: 37ºC incubation oven, waterbath, microwave or hot plate.

Storage: Some Components Require Refrigerator and Freezer Storage.

 

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