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AP Biology Investigation #11: Transpiration


Innovating Science™

Students will study the process of transpiration/transpiration pull and the role it plays in water/
nutrient movement in plants. They will study the role of stomata in relation to the transpiration
process using a potometer to examine the rate of transpiration in a bean seedling under a select set of environmental conditions. They will also examine the location/density of stomata from a leaf surface by preparing a stomatal peel. Lastly students will design and conduct an experiment to show how altering an environmental condition may affect the rate of transpiration.

Materials provided for 32 students in eight lab groups.

Meets AP Science Practices 1, 2, 4, 6, and 7, and Big Idea 4. 

Kit Includes:
8 pc Clear tubing, 12” 8 Tubing clamps
8 Pipettes, 1ml 8 Syringes, 10ml
8 Planting trays, 6 cell 1 bag Potting soil
1 pkg Bean seeds 1 tube Petroleum jelly
1 btl Nail polish 1 pkg Microscope slides

Materials Needed but not Supplied:
Calculators Microscopes
Distilled or deionized water Clear cellophane tape
Razor blades or scalpels Laboratory stands w/clamps
Light source Large shallow trays/containers
Miscellaneous plant leaves
Optional: Exact materials required will vary depending on design of student experiments. Some common item may include:
Fan Large plastic bags
Thermometers Spray bottles

DOT: Small quantity exemption 173.4
THIS PACKAGE CONFORMS TO 49 CFR 173.4 for domestic highway or rail transport only

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