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AP Biology Investigation #4: Diffusion & Osmosis


Innovating Science™

Students will study the movement of water and nutrients across a cell membrane and observe osmosis in living tissue. They will then investigate the relationship between surface area and volume as it relates to cells and diffusion. They will also examine the concept of molarity and how it relates to osmotic potential and the movement of water. Students will also be able to explain how cell size and shape affect the overall rate of nutrient intake and water elimination. Students will also use plant tissue to determine the molarities of unknown solutions based on the direction and degree of water movement.

This kit contains enough materials for 8 groups. Teacher’s manual and Student Study Guide copymasters are included.

Meets AP® Science Practices 2, 4, and 5, and Big Idea 2.

Kit Includes:
45g Agar
15ml Bromothymol blue concentrate 40 pc. Dialysis tubing
2x25ml 2.0M Hydrochloric acid 40 Plastic cups, 7oz
8 Agar block casting trays Microscope slides, pkg/72
8 Plastic cups, 5oz Coverslips
8 Plastic knives Cork borer
8 Plastic stirrers
8 Metric rulers
Sucrose QuickSolution (to make 1L of 1.0M solution)
Sodium chloride QuickSolution (to make 1L of 1.0M solution)
Glucose QuickSolution (to make 1L of 1.0M solution)
Ovalbumin QuickSolution (to make 1L of 5.0% solution)
Food coloring set (red, blue, yellow, green)
Sucrose QuickSolution set to make:
1L of 0.2M solution (Solution #3)
1L of 0.4M solution (Solution #2)
1L of 0.6M solution (Solution #5)
1L of 0.8M solution (Solution #1)
1L of 1.0M solution (Solution #4)
1L distilled water (Solution #6)

Materials Needed but not Supplied:
Paper towels
Distilled or deionized water
Electronic balance
Large onion
Fine-point forceps/tweezers
Potatoes, sweet potatoes, or yams

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