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AP Biology Investigation #7: Cell Division: Mitosis & Meiosis

APB3707

Innovating Science™

Students will study the cellular steps involved in DNA replication and cell division in both mitosis
and meiosis. This investigation will allow students to examine the stages of mitosis in the preparation of plant root tips. Crossing over in meiosis will also be investigated through the use of Sordaria cultures.

Materials provided for 32 students in eight lab groups.

Meets AP Science Practices 1, 5, 6, and 7, and Big Idea 3. 

 

Materials Included in the Kit

  • Sordaria agar, 200ml
  • Sordaria mating agar, 200ml
  • Disposable scalpels, sterile
  • 10 Petri dishes, sterile
  • 20 Disposable inoculating loops, sterile
  • 8 Forceps
  • 8 Plastic pipettes
  • 16 Plastic cups
  • 8 Microscope slides
  • Coverslips
  • Sand, 500g
  • Hydrochloric acid, 6.0M, 30ml
  • Toluidine blue, 1%, 30ml

Coupon redeemable for:

  • Sordaria, wild type
  • Sordaria, tan mutant

Materials Needed but not Supplied:

  • Microscopes
  • Distilled water
  • Microwave or hot waterbath
  • Garlic cloves

Optional:

  • Lectin
  • pH buffers
  • NaCl
  • Plant hormones (e.g. indole acetic acid, kinetin)
  • Herbicides
  • Any other materials students may think might affect the rate of mitosis in garlic root tips

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