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Thin-Layer Chromatography Kit: Analysis of Plant Pigments


Thin-layer chromatography (TLC) has become an important analytical technique since methods were devised to insure the uniform application of absorbents on glass or plastic sheets. This method is more efficient than paper chromatography because of its speed, greater sensitivity, resolving power and ease in handling. Using the precoated plastic chromatographic sheets and the concentrated solutions supplied in this kit, the most vivid demonstration of chromatography is assured. For the first time students can accomplish a dramatic TLC demonstration in a single classroom period without the difficulty of preparing slurries and plating glass strips. Each student can run two chromatograms, one using concentrated chlorophyll pigment, the other using concentrated carotenoid pigment. These pigments have been extracted by a method yielding extremely dark pigments showing excellent separation bands of the Carotenoids, Xanthophylls, Phaeophytins and Chlorophylls. This extract retains its color and stability indefinitely. Full TLC background information and instructions included.

CONTENTS: (Sufficient for 48 analyses) 60 TLC chromatogram strips, 24 Developing chambers and caps, 1 Vial concentrated carotenoid pigment, 1 Vial concentrated chlorophyll pigment, 1 Vial micro-applicators, 2 Bottles developing solvent. (Sufficient for 48 analyses of plant pigments)

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