Edvotek Series 100 Experiments. For 6 gels (5 lanes each). Time required: Gel Prep - 30 min, Electrophoresis (125v) - 45 min., Staining - 15 min, Destaining - 20 min.
Biotechnology-based diagnostic tests are beginning to supplement existing medical tests. In this experiment, students determine a pedigree for a family suspected to be carriers of mutations in their p53 genes. This is followed by a diagnostic agarose gel analysis to diagnose the state of the p53 gene in individual family members. Ready-to-Load DNA stable at room temperature.
REQUIREMENTS: Horizontal Gel Electrophoresis Apparatus, D.C. power supply, automatic micropipette with tips, balance, microwave oven or hot plate, waterbath (65 C), pipet pumps or bulbs, 250 ml flasks, hot gloves and safety goggles, latex or vinyl gloves, distilled or deionized water, photodocumentation system (optional), visualization system (white light), large weigh boats for destaining.
CONTENTS: Complete instructions, backround information, and study questions. Ready-to-load predigested DNA samples. UltraSpec-Agarose powder. Practice gel loading solution. Electrophoresis buffer (50x). Calibrated pipet. 100 ml graduated cylinder. Microtipped transfer pipets. InstaStain methylene blue. Methylene blue plus liquid stain. Per Kit.