Edvotek Series 100 Experiments. For 6 gels (6 lanes each). Time required: Gel prep 30 min., Electrophoresis 45 min., Staining 15 min., Destaining 20 min.
Coronary heart disease and stroke are the first and third leading causes of death, respectively, in the United States. Elevated blood cholesterol has been established as a serious risk factor for both of these conditions. A disease known as familial hypercholesterolemia (FH) causes an increase in blood levels of the "bad" form of cholesterol, known as low-density lipoprotein (LDL). In heterozygous patients, untreated FH causes a shortened lifespan, while homozygous individuals usually die before age 20 of coronary heart disease. In this experiment, a simulated genetic test for FH is demonstrated in which patients are tested for a DNA polymorphism linked to the FH gene.
REQUIREMENTS: Horizontal gel electrophoresis apparatus, D.C. power supply, automatic micropipet with tips, balance, microwave or hot plate, waterbath (65ºC), pipet pumps or bulbs, 250 ml flasks, hot gloves and safety goggles, latex or vinyl gloves, distilled or deionized water, photodocumentation system (opt), visualization system (white light), large weigh boats for destaining.
CONTENTS: Ready-to-Load™ Predigested DNA samples, UltraSpec-Agarose™ powder, Practice Gel Loading Solution, Electrophoresis buffer (50x), 1 ml pipet, 100 ml Graduated Cylinder, Microtipped transfer pipets, InstaStain™, Methylene Blue, Methylene Blue Plus™, Complete instructions, background information, and study questions.