Edvotek Series 100 Experiments. For 6 Gels (5 lanes each). Time required: Gel Prep - 30 min., Electrophoresis (125v) - 45 min., Staining - 15 min., Destaining - 20 min.
This experiment introduces students to the use of DNA fingerprinting in a paternity determination. A child's DNA is compared with his parents. (The experiment does not contain human DNA.)
REQUIREMENTS: Horizontal Gel Electrophoresis Apparatus, D.C. power supply, automatic micropipet with tips, balance, microwave oven or hot plate, waterbath (65 C), pipet pumps or bulbs, 250 ml flasks, hot gloves and safety goggles, latex or vinyl gloves, distilled or deionized water, photodocumentation system (optional), visualization system (white light), large weigh boats for destaining.
CONTENTS: Complete instructions, backround information, and study questions. Ready-to-load predigested DNA samples. UltraSpec-Agarose powder. Practice gel loading solution. Electrophoresis buffer (50x). Calibrated pipet. 100 ml graduated cylinder. Microtipped transfer pipets. InstaStain methylene blue. Methylene blue plus liquid stain. Per Kit.