Edvotek Series 100 Experiments.For 6 gels (6 lanes each). Time required: Gel Prep - 30 min, Electrophoresis (125v) - 45 min, Staining - 15 min, Destaining - 20 min.
Genetically inherited diseases in humans can be caused by a single nucleotide mutation resulting in a change. Such a substitution can affect the structure and function of a critical protein rendering it partially or completely nonfunctional. A classic example is hemoglobin in sickle cell anemia. This agarose gel based experiment demonstrates the use of a restriction enzyme that discriminates between the normal and sickle cell hemoglobin genes. Ready-to-Load DNA stable at room temperature.
REQURIRMENTS: Horizontal Gel Electrophoresis Apparatus, D.C. power supply, automatic micropipette with tips, balance, microwave oven or hot plate, waterbath (65 C), pipet pumps or bulbs, 250 ml flasks, hot gloves and safety goggles, latex or vinyl gloves, distilled or deionized water, photodocumentation system (optional), visualization system (white light), large weigh boats for destaining.
CONTENTS: Complete instructions, backround information, and study questions. Ready-to-load predigested DNA samples. UltraSpec-Agarose powder. Practice gel loading solution. Electrophoresis buffer (50x). Calibrated pipet. 100 ml graduated cylinder. Microtipped transfer pipets. InstaStain methylene blue. Methylene blue plus liquid stain. Per Kit.