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Size Determination of DNA Restriction Fragments - for 6 Gels (3 Gels per Lane).

BT104

Edvotek 100 Series. For 6 Gels (3 gels per lane). Time Required after gel preparation and electrophoresis: Staining -- 15 min. Destaining -- 30 min.

Size estimation of DNA fragments is the first step in DNA mapping. This experiment introduces the procedures for DNA fragment size determination. It includes standard DNA markers and fragments of unknown size.

REQUIREMENTS: Horizontal gel electrophoresis apparatus; D.C. power supply; visualization system (white light); automatic micropipet with tips; pipet pumps or bulbs; 250ml flasks; hot gloves and safety goggles; latex or vinyl gloves; metric rulers; distilled or deionized water; microwave oven or hot plate; photodocumentation system (optional); balance; 65°C waterbath; large weigh boats for destaining.

CONTENTS: Ready-to-load Pre-digested DNA Samples; Practice Gel Loading Solution; UltraSpec-Agarose™ Powder; Concentrated 50x Electrophoresis Buffer; 1ml Pipet; 100ml Graduated Packing Cylinder; Microtipped Transfer Pipets; Methylene Blue Plus™; DNA Blue InstaStain™; Complete instructions, background information, and study questions.

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