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Transformation Of E. Coli - With A Plasmid Containing The Gene For The Green (Gfp) Fluorescent Protein.

BT223

Edvotek Series 200 Experiments. For 10 Transformations and Controls Time Required: Transformation 45 min., Plating 5 min., Incubation overnight, Transformation efficiency 15 min.

In this experiment, transformants acquire the green (gfp) fluorescent protein containing plasmid. The gfp gene was isolated and cloned from the jellyfish Aequorea Victoria. The transformant colonies which contain the gfp protein are visibly green and will fluoresce when exposed to long wave UV light, commonly known as black light.

REQUIREMENTS: Incubation oven, 2 waterbaths, microwave oven or hot plate, automatic micropipet (5-50µl) and tips, pipet pumps or bulbs, ice, hot gloves and goggles, latex or vinyl gloves, long wave UV light, marking pens. Some components require -20ºC freezer storage.

CONTENTS: Transformation Cells, Plasmid DNA for gfp, IPTG, Ampicillin Antibiotic, Calcium Chloride, ReadyPour™ Luria Broth Agar, Luria Broth Media for Recovery, Petri plates, 60mm & 100mm, Wrapped 10 ml pipets, Sterile Calibrated Transfer Pipets, Sterile Inoculating Loops, Microtest tubes with attached caps, Toothpicks, Complete instructions, background information and study questions.

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